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Thermo Fisher pentr™/sd/d-topo vector
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Agilent technologies bl21 codonplus e. coli strain for expression of recombinant proteins
Bacterial strains and plasmids used in this study
Bl21 Codonplus E. Coli Strain For Expression Of Recombinant Proteins, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bacterial strains and plasmids used in this study
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Thermo Fisher pentr tm /sd/d-topo tm vectors
Bacterial strains and plasmids used in this study
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Thermo Fisher pentr sd/d topo cloning vector
Bacterial strains and plasmids used in this study
Pentr Sd/D Topo Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher pentr/sd/d-topo vectors
Bacterial strains and plasmids used in this study
Pentr/Sd/D Topo Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bacterial strains and plasmids used in this study

Journal: Infection and Immunity

Article Title: The Fimbrial Protein FlfA from Gallibacterium anatis Is a Virulence Factor and Vaccine Candidate

doi: 10.1128/IAI.00059-13

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: The bacterial strains and plasmids used in the present study are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristics a Source or reference Strains G. anatis 12656-12 Wild-type strain; biovar 4, isolated in 1981 (Denmark) 51 Δ flfA mutant flfA deletion mutant of G. anatis 12656-12; Km r This study F149 T Wild-type strain; isolated in 1979 (Denmark) 2 10672/6 Wild-type strain; biovar 1 (Denmark) 2 4895 Wild-type strain; biovar 4 (Mexico) 52 7990 Wild-type strain; biovar 1 (Mexico) 52 Avicor Wild-type strain; biovar 3 (Mexico) 52 CCM5995 Wild-type strain; biovar 20, isolated in 1978 (Czech Republic) 2 IPDH 697-78 Wild-type strain; biovar 15, isolated in 1978 (Germany) 2 E. coli TOP10 E. coli host strain for routine cloning Invitrogen DH5α E. coli host strain for routine cloning Invitrogen BL21 CodonPlus E. coli strain for expression of recombinant proteins Stratagene Plasmids pENTR/SD/D-TOPO Directional cloning vector for entry to the Gateway System Invitrogen pDEST17 Gateway Destination vector (Amp r , N-terminal His 6 fusion tag) Invitrogen pDEST17 flfA FlfA expression vector (Amp r , N-terminal His 6 fusion tag) This study pBluescript II KS(+) General purpose cloning vector (Amp r ) Stratagene pUC4K Vector carrying the Km r cassette from Tn 903 53 pBSΔ flfA pBluescript II KS(+) containing flanking regions of the flfA gene (Amp r ) This study pBSΔ flfA -Km r Plasmid for flfA deletion with flanking regions of the gene cloned on either side of the Km r cassette from pUC4K into pBluescript II KS(+) (Amp r Km r ) This study Open in a separate window a Amp r , ampicillin resistance; Km r , kanamycin resistance; His, histidine.

Techniques: Plasmid Preparation, Isolation, Mutagenesis, Clone Assay, Expressing, Recombinant